Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Effect of Corynoline Isolated from Corydalis bungeana Turcz on Lipopolysaccharides-Induced Sepsis In vivo and In vitro

Zhi-biao He¹, Ping Chen², Zhen-yu Peng¹, Li-yan Jin³

¹Department of Emergency; ²Department of Pulmonary Medicine; ³Department of Anesthesia, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, PR China.

For correspondence:- Li-yan Jin Email: lyanjmsu@126.com Tel:+86073185295970

Received: 11 September 2013 Accepted: 18 November 2013 Published: 25 January 2014

Citation: He Z, Chen P, Peng Z, Jin L. Effect of Corynoline Isolated from Corydalis bungeana Turcz on Lipopolysaccharides-Induced Sepsis In vivo and In vitro. Trop J Pharm Res 2014; 13(1):81-86 doi: 10.4314/tjpr.v13i1.12

© 2014 The authors.
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Abstract

Purpose: To investigate the protective effect of corynoline isolated from Corydalis bungeana Turcz on lipopolysaccharides (LPS)-induced sepsis, and determine the possible mechanism of anti-sepsis effect of the isolated corynoline.

Methods: Corynoline was extracted by column chromatography. LPS (100 ng/mL) was used to induce the release of TNF-α and IL-6 in RAW 264.7 cells, and the isolated corynoline was added. ELISA method was used to determine the levels of TNF-α and IL- 6. Furthermore, sepsis in mice was established by injection of LPS (2 mg/kg, i.v.), and the levels of TNF-α and IL-6 in plasma were determined by ELISA method. For survival rate test, LPS (15 mg/kg, i.v.) and heat-killed E. coli (1.0 ×10¹¹ CFU/kg, i.v.) were used to establish sepsis in mice model, and the mice were observed in 7 days.

Results: The results indicate that corynoline significantly elevated the survival rate of septic mice induced by LPS and heat-killed E. coli, in a dose-dependent manner (p < 0.05). Corynoline decreased the release of TNF-α and IL-6 induced by LPS, in a dose-dependent manner (p < 0.05).

Conclusion: Treatment with corynoline significantly inhibits the mortality of LPS-induced septic mice, and the mechanism of action is probably related to the decrease of TNF-α and IL-6 release. Thus corynoline has the potential to be developed as an effective and safe drug for treating sepsis.

Keywords: Corynoline, Corydalis bungeana, Sepsis, Lipopolysaccharides, TNF-^5;, IL-6